WaxFree Nucleic Acids Extraction

WaxFreeTM Technology

Formalin-fixed and paraffin-embedded (FFPE) tissue samples are routinely generated in hospitals as valuable source for molecular and histopathological diagnosis of diseases like cancers. However, DNA or RNA of FFPE samples undergoes moderate to severe degradation during the formalin fixation process and usually has poor binding efficiency to beads or column filters. Most commercially available DNA/RNA extraction kits use the binding/elution process and lose 40-60% of nucleic acids because of inefficient binding and incomplete elution.

TrimGen’s WaxFreeTM technology is a non-binding, non-elution method to efficiently extract DNA or RNA from tissue and eliminate the loss seen in traditional column or bead extraction methods. This simple extraction technology takes less than two hours and achieves maximal yield of DNA or RNA with optimized conditions for downstream applications such as PCR amplification, RT-PCR and NGS.

WaxFreeTM DNA Extraction Process and Example Results:

 

 

WaxFreeTM RNA Extraction Process and Example Results:

The technology uses a special chemistry and a resin and enzyme mix to remove inhibitors and release the nucleic acids into the extract. In a side-by-side comparison of 10 methods for nucleic acids extraction from FFPE samples, Okello et al. demonstrated that TrimGen'sWaxFreeTM method recovered the highest yields of amplifiable  DNA1.

1.     Okello, J.B., et al. (2010). Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues. Anal Biochem, 400:110.